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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Evodiamine Induces Apoptosis and Inhibits Migration of HCT-116 Human Colorectal Cancer Cells
doi: 10.3390/ijms161126031
Figure Lengend Snippet: Migration ability, expression of PGI/AMF, JAK2/STAT3, and MMP3 were detected in HCT-116 cells treated with EVO. ( A ) Migration potential was assessed by Transwell assay after cells were treated by agents. Cells were seeded in Transwell insert upper part incubated with 10 μg/mL PGI/AMF ( b ) and with 10 μg/mL PGI/AMF + 6 μmol/L EVO ( c ) for 28 h, normal cells as the control group ( a ) (original magnification, 200×); ( B ) AMF was detected by ELISA after cells were treated with various concentrations of EVO for 6 h; ( C – F ) Cells were incubated with 6 μmol/L EVO for 2, 4, and 6 h, and the expression of PGI, MMP3 and JAK2/STAT3 was assessed. Relative expression ratio was calculated with Gray-scan value for ( D , F ). Data represent the results of three independent experiments (mean ± SD). Statistically different compared with the control (0 μmol/L of EVO) ( * p < 0.05; ** p < 0.01).
Article Snippet: The
Techniques: Migration, Expressing, Transwell Assay, Incubation, Control, Enzyme-linked Immunosorbent Assay